Rapid detection of ERG11 polymorphism associated azole resistance in Candida tropicalis
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چکیده
منابع مشابه
Azole Resistance and ERG11 464 Polymorphism in Oral Candida albicans Clinical Strains Isolated in Sardinia
The in vitro activity of three different azoles (fluconazole, FLC, voriconazole, VRC and ketoconazole, KTC) was determined and correlated with the single nucleotide polymorphisms (SNPs) in a “hot spot” region of the ERG11 gene in a collection of 52 arbitrarily selected C. albicans strains isolated from Sardinian subjects with oropharyngeal candidiasis. Among the strains evaluated, 23.1% were re...
متن کاملContribution of clinically derived mutations in ERG11 to azole resistance in Candida albicans.
In Candida albicans, the ERG11 gene encodes lanosterol demethylase, the target of the azole antifungals. Mutations in ERG11 that result in an amino acid substitution alter the abilities of the azoles to bind to and inhibit Erg11, resulting in resistance. Although ERG11 mutations have been observed in clinical isolates, the specific contributions of individual ERG11 mutations to azole resistance...
متن کاملNucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates.
One of the mechanisms of Candida albicans resistance to azole drugs used in antifungal therapy relies on increased expression and presence of point mutations in the ERG11 gene that encodes sterol 14α demethylase (14DM), an enzyme which is the primary target for the azole class of antifungals. The aim of the study was to analyze nucleotide substitutions in the Candida albicans ERG11 gene of azol...
متن کاملMechanisms of azole resistance in 52 clinical isolates of Candida tropicalis in China.
OBJECTIVES To explore the mechanisms underlying azole resistance in clinical isolates of Candida tropicalis collected in China by focusing on their efflux pumps, respiratory status and azole antifungal target enzyme. METHODS Fifty-two clinical isolates of C. tropicalis were collected from five hospitals in four provinces of China and antifungal susceptibility tests were performed. Rhodamine 6...
متن کاملMechanisms of azole resistance in a clinical isolate of Candida tropicalis.
Azole resistance has been insufficiently investigated in the yeast Candida tropicalis. Here we determined the molecular mechanisms responsible for azole resistance in a clinical isolate of this pathogenic yeast. Antifungal susceptibility testing performed by a disk diffusion method showed resistance or markedly decreased susceptibility to azoles, which was confirmed by determination of MICs. Co...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2021
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0245160